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Table 3 Results of receiver operating chraracteristics (ROC) analysis

From: Toxoplasma gondii and Neospora caninum infections in South American camelids in Switzerland and assessment of serological tests for diagnosis

ELISA Kit Optimized ELISA cut-off AUC
(95% CI)
% Relative sensitivity (95% CI) % Relative specificity (95% CI) PPV % (95% CI) NPV % (95% CI)
TOXO-MS ≥ 36.2 S/P% 0.97 (0.94–1.00) 94.5 (87.6–98.2) 97.9 (92.6–99.7) 97.7 (92.0–99.3) 94.9 (88.5–99.4)
NCS-MS ≥ 34.9 S/P% 0.96 (0.91–1.01) 89.3 (71.8–97.7) 100.0 (95.4–NaN) 100 (86.9–100) 96.3 (88.8–NaN)
NCC ≤ 78.6 S/N% 0.97 (0.92–1.01) 92.9 (76.5–99.1) 97.4 (91.0–99.7) 92.9 (77.7–99.1) 97.4 (90.5–99.7)
  1. Notes: Relative accuracies of commercial ELISA kits used for detection of antibodies against Toxoplasma gondii (i.e. ID Screen® Toxoplasmosis Indirect ELISA) and N. caninum (i.e. ID Screen® Neospora caninum Indirect Multi-species ELISA and ID Screen® Neospora caninum Competition ELISA) in South American camelids, in relation to N. caninum and T. gondii immunoblots
  2. Abbreviations: TOXO-MS, ID Screen® Toxoplasmosis Indirect; NCS-MS, ID Screen® Neospora caninum Indirect Multi-species ELISA; NCC, ID Screen® Neospora caninum Competition; S/P%, sample-to-positive ratio; S/N%, competition percentage; AUC, area under the ROC curve; CI, confidence interval; TP, true positives; FP, false positives; TN, true negatives; FN, false negatives; PPV, positive predictive value; NPV, negative predictive value; NaN, upper limit of the confidence interval could not be computed