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Fig. 5 | Parasites & Vectors

Fig. 5

From: Investigation of the antigenicity and protective efficacy of Leishmania promastigote membrane antigens in search of potential diagnostic and vaccine candidates against visceral leishmaniasis

Fig. 5

Sandwich ELISA for the cytokine assay. In vitro cytokine production is shown by the PBMCs of VL-treated and healthy individuals in ELISA upon stimulation with LAg and by the PBMCs of treated VL patients with electroeluted 31, 34, 51, 63, 72 and 91 kDa proteins for 96 h, (n = 6). Unstimulated PBMCs were included here as control. Cytokines were measured by ELISA for IFN-γ production (a), IL-12 production (b), IL-10 production (c), TGF-β production (d), IFN-γ/IL-10 ratio (e) and IFN-γ/ TGF-β ratio (f). Unpaired Studentʼs t-tests were used to analyse the data

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Parasites & Vectors

ISSN: 1756-3305

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