Fig. 3

PfArp4 knockdown altered the global transcriptome. a The experimental design of parasite treatment and sampling for RNA-seq analysis. The highly synchronized parasites of PfArp4-Ty1-Ribo clone (7A) were harvested in the second cycle after exposure to GlcN for one cycle. Sampling: ring (R2, 8–12 h), trophozoite (T2, 28–32 h), schizont (S2, 40–44 h). b Scatterplots showing the comparative transcriptomes of PfArp4-Ty1-Ribo line with versus without GlcN at R, T, and S stage, respectively. Fold change (FD) ≥ 2 are used to differentiate the upregulated or downregulated genes. c Heatmap showing the fold change (log2) of gene expression upon PfArp4 knockdown across the R, T and S stage, respectively. d Enriched Gene Ontology (biological processes) terms for the genes differentially expressed in PfArp4 knockdown line at ring stage