Fig. 3

Extraction method and copy number comparison. Cq values of promastigotes isolated from culture (black symbols) and sand fly midguts (grey symbols) that were extracted with a commercial column extraction (‘column’) or crude high-salt extraction buffer (‘crude’) and subjected to JW kDNA and SL RNA qPCRs. Each symbol presents the assay result for a standardized concentration of promastigotes that was used for the comparisons. Abbreviation: Cq, quantification cycle