Fig. 4

Performance of the SL RNA qPCR on a serial dilution of sand flies spiked with promastigotes. a Standard curve with linear regression and qPCR efficiency. The open symbols depict all concentrations that were detected by the assay, while the filled symbols are the parasite concentrations that show a linear correlation. b Inter-run variability of the SL RNA qPCR in two replicates, analyzed by Pearson’s correlation and linear regression analysis. Dotted lines indicate the 95% confidence intervals. Abbreviation: Cq, quantification cycle