Table 1 Cq values of the SL RNA qPCR assay with and without reverse transcriptase enzyme
With reverse transcriptase (RNA + DNA) | Without reverse transcriptase (DNA) | Cq difference | % DNA |
|---|---|---|---|
15.0 | 19.7 | 4.8 | 3.7 |
27.4 | 30.8 | 3.4 | 9.8 |
23.6 | 27.7 | 4.2 | 5.6 |
14.8 | 21.5 | 6.7 | 1.0 |
14.0 | 19.2 | 5.2 | 2.7 |
15.6 | 20.9 | 5.3 | 2.6 |
16.3 | 20.7 | 4.3 | 5.0 |
13.7 | 20.2 | 6.5 | 1.1 |
16.1 | 20.4 | 4.3 | 5.0 |
14.4 | 20.7 | 6.3 | 1.3 |
Mean ± SD |  | 5.1 ± 1.1 | 3.8 ± 2.7 |