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Fig. 3 | Parasites & Vectors

Fig. 3

From: First record of besnoitiosis caused by Besnoitia bennetti in donkeys from the UK

Fig. 3

Immunoblot reactions against non-reduced Besnoitia besnoiti tachyzoite or bradyzoite antigens of donkeys from UK suspected of being infected with Besnoitia bennetti. Antigen bands selected for scoring the immunoblot reactions are marked (<). Ten tachyzoite and 10 bradyzoite antigen bands were recorded due to the absence of cross-reactivity to Sarcocystis spp., N. caninum or T. gondii [10]. Apart from one sample (tissue fluid, donkey no. 8, tachyzoite antigen), all tested samples showed positive reactions with more than 3 of the selected 10 antigen bands (see text for details on the number of recorded bands per sample). Lane 1: tissue fluid from donkey no. 8 (Table 1); Lanes 2–5: sera from donkey nos. 3, 4, 5 and 7 (Table 2). Abbreviations: Mr, relative molecular weight; N, negative; P, positive control donkey sera obtained from a previous study in the USA [7]

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