Fig. 3

Influence of Asteglut1, Asteglutx, Asteglut3 and Asteglut4 on vector competence. a–d Knocking down efficiency of Asteglut1, Asteglutx, Asteglut3 and Asteglut4. Relative expression levels of Asteglut1, Asteglutx, Asteglut3 and Asteglut4 were normalized to that in dsGFP controls. Ribosomal gene s7 used as an internal control. Error bars indicate standard error of the mean (n = 10). Results from one of three independent experiments are shown. Significance was determined by Student’s t-test. e-h Oocyst number in dsRNA-treated mosquitoes. Each dot represents an individual mosquito and horizontal lines represent the medians. Results from one of three independent experiments are shown. Significance was determined by Mann-Whitney test. i Specificity of dsAsteglut1 treatment. Relative expression level of Asteglut1, Asteglutx, Asteglut3 and Asteglut4 in dsAsteglut1 treated mosquitoes were normalized to that in dsGFPs. Error bars indicate mean ± SEM (n = 10). Results from one of three independent experiments are shown. Significance (a–c, d, i) was determined by Student’s t-test (for details, see Additional file 4: Text S1). Significance (e–h) was determined by Mann-Whitney test (for details, see Additional file 4: Text S1). Abbreviations: NS, non-significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001