Fig. 4

Influence of Asteglut1 on glucose transportation in the mosquito midgut. The relative concentration of glucose (a, c) and trehalose (b, d) in the midgut and hemolymph of GFP and Asteglut1 dsRNA- injected An. stephensi 24 h prior to (− 24 h), just before (0 h) and 24 h post-blood-feeding (24 h), respectively. Sugar concentration was normalized to genomic DNA extracted from midgut or hemolymph cells. Error bars indicate mean ± SEM (n = 5). Significance was determined by Student’s t-test (for details, see Additional file 4: Text S1). Abbreviation: NS, non-significant. **P < 0.01