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Fig. 7 | Parasites & Vectors

Fig. 7

From: Initiator and executioner caspases in salivary gland apoptosis of Rhipicephalus haemaphysaloides

Fig. 7

Biological effects of RNAi of Rhipicephalus haemaphysaloides RhCaspases 7, 8 and 9 in vivo. a Images comparing ticks inoculated with dsRhCaspases 7, 8 and 9 with control groups on day 6 of feeding. dsRhCaspase 9 inhibited the blood-feeding process and resulted in ticks smaller than the controls. b Confirmation of RhCaspase 7, 8 and 9 silencing using RT-qPCR. Total RNA was extracted on feeding day 5 from female ticks injected with dsRNA. Bars represent mean relative expression of RhCaspase 7, 8 and 9 genes; error bars represent standard error. ***P < 0.001, based on two-tailed Student’s t-tests. c Positive TUNEL staining decreased after RNAi of RhCaspases 7, 8 and 9, and DNA was stained with DAPI. Scale-bars: c, 50 μm

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