Fig. 1
From: Sirolimus enhances the protection achieved by a DNA vaccine against Leishmania infantum
Graphical representation of the experimental assay. The study comprised a large experiment with a total of 34 Syrian golden hamsters randomly distributed in 4 groups: NI (non-infected group, n = 4), C (PBS-inoculated control group, n = 10), V (DNA vaccinated group, n = 10), and S + V (sirolimus-treated and DNA vaccinated group, n = 10). Hamsters received sirolimus or a PBS solution daily from one day prior to the first vaccination for 6 consecutive weeks. Three vaccine doses were administered, leaving a 2-week period between them. C, V and S + V animals were challenged two weeks after receiving the last vaccination dose and all hamsters were sacrificed 5 weeks later