Fig. 4
From: Sirolimus enhances the protection achieved by a DNA vaccine against Leishmania infantum
Cytokine expression in spleen samples at the end of the study. IFN-γ, TNF-α, NOS2, IL-10, IL-21, FOXP3, and TGF-β expression was determined by RT-qPCR using SYBR Green as the detection chemistry. NI: non-infected group (n = 4); C: PBS control group (n = 10); V: DNA vaccinated group (n = 10); S + V: sirolimus-treated and DNA vaccinated group (n = 10). NI group was taken as a reference group, with a value of 1. ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001**** P < 0.0001