Fig. 1

RPA of the G. duodenalis β-giardin gene. a RPA primers amplify nts 796–978 of GenBank X85958: binding sites of primers (clear boxes) and probe (shaded box) with modifications of the probe (5’ FAM, internal THF, 3’ block) and the reverse primer (5’ biotin); the reverse primer is depicted on the corresponding sequence of the sense strand. b Identification of predicted amplification products by gel electrophoresis. See Methods for the derivation of the two amplicons. c Presence of the double-labelled RPA product detected visually on the lateral-flow cassette from trophozoite and cyst DNA. Abbreviations: Mk, DNA size marker; nts, nucleotides; THF, tetrahydrofuran