Fig. 5

Phylogenetic positioning of Trypanosoma (Megatrypanum) trinaperronei n. sp. a Phylogenetic tree based on concatenated gGAPDH and SSU rRNA sequences from Megatrypanum trypanosomes and species representative of all other major clades of Trypanosoma, using trypanosomatids of other genera as outgroups. Major phylogenetic clades were collapsed, and the clade Megatrypanum was highlighted. bMegatrypanum clade showing TthI and TthII lineages formed by several genotypes. Trypanosoma trinaperronei n. sp. was assigned to genotype TthII H, sister to TthII C, which comprised the German Trypanosoma sp. D30 of fallow deer and the Croatian TC2 of red deer, altogether forming a clade exclusive of deer trypanosomes from South and North America, and Europe. A concatenated alignment of 1575 characters was employed for maximum likelihood (ML) and Bayesian inference (BI); the numbers at nodes refer to ML/B support values derived from 500 replicates