Fig. 7

Light and scanning electron microscopy (SEM) of Trypanosoma trinaperronei n. sp. co-cultured with insect cells at 25 °C. Giemsa-stained (a–c) forms of a log-phase (5 days) culture (a) exhibiting flagellates adhered by their flagella forming rosettes, and detached epimastigotes. b Mid-log (7 days) cultures showing long and thin epimastigotes. c Stationary culture (10 days) exhibiting epimastigotes, transition forms between epi- and trypomastigotes (arrow heads), and unique bell-shaped metacyclic trypomastigotes. A typical metacyclic was enlarged in (d). SEM of mid-log cultures (7 days) showing epimastigotes of variable length and width (e–j) including forms with well-developed undulant membranes (f, j), transition forms (f–h), and bell-shaped metacyclic trypomastigotes (f, g, k). Abbreviations: nucleus, n; kinetoplast, k; flagellum, f; undulating membrane, um; epimastigote, E; trypomastigote, T; metacyclic trypomastigotes, mT. Scale-bars: a–k, 10 µm