Fig. 2

Quality controls of the T. cruzi growth inhibition assay (a, b), toxicity assay with Vero cells (c, d) and toxicity assay with HepG2 cells (e, f). Z’-values for each of the rounds launched are represented on the left (a, c, e); dashed line marks the 0.5 threshold. IC₅₀ and TC₅₀ values of the reference drugs BNZ and DTX are represented on the right (b, d, f); continuous lines indicate the average values, whereas the dashed lines indicate ± 3 SD limits