Fig. 4

Illustration of stage-specific transcripts present in ten clusters identified with hierarchical clustering and intra-cluster variability evaluation. a The distribution of stage-specific transcripts within 10 transcript clusters. Mean log2-scaled read mapping counts are plotted per cluster and life stage, shades represent standard deviation. b Gene ontology terms enrichment for ten stage-specific clusters, including the cluster of “housekeeping gene” transcripts in the common transcriptome assembly of the five tick stage-specific libraries (Cluster 11). The colour and size of data points indicate − log10 values of Benjamini/Hochberg FDR-corrected p-values and fold enrichment compared to the whole set of transcripts, respectively