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Table 2 Genes used in reference gene validation assay. The genes were selected based on validation in the referenced studies

From: Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle

Gene

Annotation

Reference

Query sequence

I. ricinus homologue sequence

EF1É‘

Elongation factor 1α

[57, 58]

GU074814.1

MN728895

ferritin

Ferritin (somatic)

[112]

AY277906.1

MN728896

GAPDH

Glyceraldehyde 3-phosphate dehydrogenase

[57,58,59]

XM_002434302.1

MN728904

H3F3A

H3 histone family 3A

[58]

XM_002399526.1

MN728905

RpL13A

Ribosomal protein L13A

[59]

XM_002436237.1

MN728901

ppiA

Cyclophilin-type peptidylprolyl cis-trans isomerase A

[58]

XM_002407873.1

MN728903

RpL32

Ribosomal protein L32

[57]

XM_002399465.1

MN728898

rpl4

Ribosomal protein L4

[58]

XM_002402278.1

MN728900

rps4

Ribosomal protein S4

[59]

DQ066214.1

MN728897

sdha

Succinate dehydrogenase complex subunit A

[57]

XM_002408156.1

MN879329

TBP

TATA-box binding protein

[58, 59]

XM_002402081.1

MN879330

TUBB

β-tubulin

[57, 58]

GQ411364

MN728899

v-ATPase

Vacuolar-type H[+]-ATPase

[57]

XM_002413524.1

MN728902