Fig. 3From: Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditumVenn diagram depicting the distribution of positive samples detected by molecular and serological assays. a Molecular identification of filariae and associated Wolbachia using the multiplex approach. b Comparison between molecular diagnosis and heartworm antigen detection before and after heat pre-treatment of seraBack to article page