Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditum

Table 2 PCR/sequencing primers developed in this study, their characteristics and conditions

System name	Target gene	Primer name	Sequence (5′-3′)	Amplicon size (bp)	Tm (°C)	Specificity
Pan-fil cox1 PCR	cox1	Fwd.957	ATRGTTTATCAGTCTTTTTTTATTGG	509	52.0	Filariae
Pan-fil cox1 PCR	cox1	Rwd.1465	GCAATYCAAATAGAAGCAAAAGT	509	52.0	Filariae
Wol ftsZ PCR	ftsZ	Wol.ftsZ.363.f	GGRATGGGTGGTGGYACTGG	560	59.5	Wolbachia
Wol ftsZ PCR	ftsZ	Wol.ftsZ.958.r	GCATCAACCTCAAAYARAGTCAT	560	59.5	Wolbachia

ISSN: 1756-3305