Fig. 1

Agarose gel (2.5%) electrophoresis of amplified gDNA products in several mosquitoes using multiplex PCR. The arrows indicate a common band of 368 bp, a band of 232 bp corresponding to the susceptible allele F1534, a band of 180 bp corresponding to the resistant allele C1534, a band of 133 bp corresponding to the susceptible allele V410, a band of 113 bp corresponding to the resistant allele L410, a band of 102 bp corresponding to the susceptible allele V1016, and a band of 82 bp corresponding to the resistant allele I1016. Lane 1: molecular weight marker (25 bp DNA Ladder); Lane 2: a resistant homozygous (C1534/C1534), heterozygous (V410/L410) and heterozygous (V1016/I1016) individual; Lane 3: a resistant homozygous individual (C1534/C1534), resistant homozygous (L410/L410) and resistant homozygous (I1016/I1016); Lane 4: a resistant homozygous individual (C1534/C1534), heterozygous (V410/L410) and resistant homozygous (I1016/I1016); Lane 5: a resistant homozygous individual (C1534/C1534), susceptible homozygous (V410/V410) and heterozygous (V1016/I1016); Lane 6: a resistant homozygous individual (C1534/C1534), heterozygous (V410/L410) and heterozygous (V1016/I1016); Lane 7: a triple susceptible homozygous individual (V410/V410, V1016/V1016 and F1534/F1534)