Fig. 2

Transcriptional levels of the Hc-tgh-2 gene in eight developmental stages of Haemonchus contortus. The relative quantities (compared with egg, egg = 1) are shown as mean values (± standard error of the mean, SE). Abbreviations: Egg, eggs; L1, first-stage larvae; L2, second-stage larvae; iL3, infective third-stage larvae; L4F, females of fourth-stage larvae; L4M, males of fourth-stage larvae; AdF, adult females; AdM, adult males. The statistical analysis showed that ANOVA, F_{(7, 16)} = 20.11002, P < 0.0001. Egg vs L1, P = 0.9994; Egg vs L2, P = 0.6577; Egg vs iL3, P < 0.0001; Egg vs L4F, P > 0.9999; Egg vs L4M, P > 0.9999; Egg vs AdF, P > 0.9999; Egg vs AdM, P > 0.9999; L1 vs L2, P = 0.9090; L1 vs iL3, P < 0.0001; L1 vs L4F, P = 0.9991; L1 vs L4F, P = 0.9997; L1 vs AdF, P = 0.9990; L1 vs AdM, P > 0.9999; L2 vs iL3, P < 0.0001; L2 vs L4F, P = 0.6399; L2 vs L4F, P = 0.6924; L2 vs AdF, P = 0.6358; L2 vs AdM, P = 0.7419; iL3 vs L4F, P < 0.0001; iL3 vs L4M, P < 0.0001; iL3 vs AdF, P < 0.0001; iL3 vs AdM, P < 0.0001; L4F vs L4M, P > 0.9999; L4F vs AdF, P > 0.9999; L4F vs AdM, P > 0.9999; L4M vs AdF, P > 0.9999; L4M vs AdM, P > 0.9999; AdF vs AdM, P > 0.9999. There were significant differences between stages indicated by different capital letters (a, b) (P < 0.01)