A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G

Table 1 List of the primers used in this study

Primers used for	Name of primer	Nucleotide sequence (5′–3′)	Reference
Amplification and DNA sequencing of domain II	AedIIF	AGACAATGTGGATCGCTTCC	This study
	AedIIR	GGACGCAATCTGGCTTGTTA	This study
	IIP_F	GGTGGAACTTCACCGACTTC	Yanola et al. [43]
	IIS6_R	GGACGCAATCTGGCTTGTTA	Yanola et al. [43]
Amplification and DNA sequencing of domain III	AedIIIF	AAC GTT CAA GGG CTG GAT CC	This study
Amplification and DNA sequencing of domain III	AedIIIR	TTC GAG CCC ATC TTT TTC AT	This study
Amplification and DNA sequencing of domain IV	5380F1	CCAAAGGTATCCGAACGTTGCTG	Chang et al. [36]
Amplification and DNA sequencing of domain IV	5380R1	CCGATCATCAACTCACCAGAAAC	Chang et al. [36]
Allele-specific PCR for S989P	AedIIF	AGACAATGTGGATCGCTTCC	This study
	AedIIR	GGACGCAATCTGGCTTGTTA	This study
	PPF	GGCGAGTGGATCGAAC	This study
	SSR	GCATACAATCCCACATGGA	This study
Allele-specific PCR for V1016G	AedIIF	AGACAATGTGGATCGCTTCC	This study
	AedIIR	GGACGCAATCTGGCTTGTTA	This study
	VVF	TCCCACTCGCACAGGT	This study
	GGR	GGCTAAGAAAAGGTTAAGTC	This study
PCR-RFLP for F1534C/L and T1520I	AekdrF	TGGGAAAGCAGCCGATTC	Kushwah et al. [15]
PCR-RFLP for F1534C/L and T1520I	AekdrR	CCTCCGTCATGAACATTTCC	Kushwah et al. [15]

ISSN: 1756-3305