Table 1 Proteins identified by 2D-DIGE/mass spectrometry as differentially expressed between intestinal epithelial cells of infected mice versus rIL-25-treated animals and exposed to metacerariae of Echinostoma caproni
Spot | Protein | Species | Expression | MW (kDa) Expected/Observed | Isoelectric point Expected/Observed | Cellular role | Location | Score | Coverage (%) | Peptides |
|---|---|---|---|---|---|---|---|---|---|---|
133 | Enolase 1B | Mus musculus | − 1.4 | 47/116 | 6.37/6.37 | Glycolysis | Cyt | 45.44 | 66.59 | 8 |
Plasminogen activation | PM | |||||||||
Ornithine metabolism | Mit | |||||||||
146 | Enolase 1B | Mus musculus | + 1.9 | 47/112 | 6.37/7.17 | Glycolyis | Cyt | 38.54 | 45.16 | 112 |
Plasminogen activation | PM | |||||||||
Ornithine metabolism | Mit | |||||||||
148 | Enolase 1B | Mus musculus | + 2.0 | 47/111 | 6.37/6.67 | Glicolisis | Cyt | 108.86 | 87.33 | 176 |
Plasminogen activation | PM | |||||||||
Ornithine metabolism | Mit | |||||||||
273 | Glyceraldehyde-3-phosphate dehydrogenase | Mus musculus | + 2.4 | 35/93 | 8.44/8.44 | Glycolysis | Cyt | 32.38 | 62.76 | 29 |
291 | Lactate dehydrogenase | Mus musculus | + 1.5 | 36/88.8 | 7.61/9.26 | Pyruvate fermentation to lactate | Cyt | 16.31 | 45.48 | 29 |