Table 3 Proteins identified by 2D-DIGE/mass spectrometry as differentially expressed between intestinal epithelial cells of naïve control mice versus rIL-25-treated animals
Spot | Protein | Species | Expression | MW (kDa) Expected/Observed | Isoelectric point Expected/Observed | Cellular role | Location | Score | Coverage (%) | Peptides |
|---|---|---|---|---|---|---|---|---|---|---|
Metabolic enzymes | ||||||||||
144 | Enolase 1B | Mus musculus | + 1.2 | 47/110 | 6.37/6.37 | Glycolysis | Cyt | 89.91 | 75.81 | 112 |
Plasminogen activation | PM | |||||||||
Ornithine metabolism | Mit | |||||||||
147 | Enolase 1B | Mus musculus | − 1.5 | 47/110 | 6.37/6.75 | Glycolysis | Cyt | 37.45 | 48.62 | 21 |
Plasminogen activation | PM | |||||||||
Ornithine metabolism | Mit | |||||||||
533 | Triosephosphate isomerase | Mus musculus | − 1.9 | 32/60 | 5.56/6.28 | Glycolysis | Cyt | 25 | 45.48 | 16 |
Gluconeogenesis | ||||||||||
Structural proteins | ||||||||||
527 | Junction plakoglobin | Mus musculus | + 1.7 | 82/27 | 5.75/8.70 | Cell adhesion | Cyt | 3.33 | 3.44 | 4 |
404 | Peroxiredosin-4 | Mus musculus | − 1.9 | 31/60 | 6.67/5.90 | Redox regulation | Cyt | 10.61 | 42.34 | 8 |
Cell regulation proteins | ||||||||||
159 | Proliferation-associated 2G4 | Mus musculus | − 1.8 | 50/108 | 6.41/6.83 | Apoptotic process | Cyt | 29.09 | 41.62 | 16 |
306 | Receptor of activated protein C kinase 1 | Mus musculus | − 1.8 | 35/83 | 7.6 | Apoptotic process | PM | 13.62 | 27.76 | 7 |
Biological rhythms | Nuc | |||||||||
Translation regulation | ||||||||||