Table 1 List of strains and plasmids used

CO92

Pgm+ pCD1- pMT1+ pPCP1+, parental strain

[32]

CO92 glmS-pstS::P_trcGFP

CO92 with chromosomally inserted gfp for imaging

This study

CO92 glpD+

Defective glpD′ allele replaced with functional glpD gene from KIM6+ in the chromosome

[32]

CO92 glpD+glmS-pstS::P_trcGFP

defective glpD′ allele replaced with functional glpD gene in the chromosome, with chromosomally inserted GFP for imaging

This study

CO92gly

Defective copy of the glpD′ allele replaced with functional glpD in the chromosome, and carries glpFKX from KIM6+ on pTpGLPFKX6

This study

CO92gly glmS-pstS::P_trcGFP

CO92gly with chromosomally inserted gfp for imaging

This study

CO92 ΔhmsD::frt

ΔhmsD

This study

CO92 ΔhmsT::frt

ΔhmsT

This study

KIM6+

Pgm+ pCD1- pMT1+ pPCP1+, parental strain

[16]

KIM6+::kan^R

KIM6+ with chromosomally inserted kanamycin resistance for co-infections

[39]

KIM6+ pAcGFP1

KIM6+ with GFP for imaging

This study

pAcGFP1

Carries AcGFP1, Cb^r

Clontech (Mountain View, CA, USA)

pTNS2

TnsABC+D specific transposition pathway, Cb^r

[33]

pKD13

Source of kanamycin resistant cassette and flanking Frt sequence

[35]

pFLP3

Recombinase, Cb^r, Tet^r

[33]

pUC18R6KT-mini-Tn7T-Km

Cloning vector for Tn7 insertion, Cb^r, Km^r

[33]

pGP-Tn7-P_trcgfpmut3

Source of P_trcgfpmut3, Cb^r

[34]

pUC18R6KT-mini-Tn7T-P_trcgfpmut3

Cloning vector for Tn7 insertion of P_trcgfpmut3, Cb^r, Km^r

This study