Oligonucleotide | Reference | Purpose | Sequence |
---|---|---|---|
p130 | [35] | Amplify kanamycin cassette and check insertion | GTGTAGGCTGGAGCTGCTTC |
p131 | [35] | Amplify kanamycin cassette and check insertion | ATTCCGGGGATCCGTCGACC |
p407/pGPTn7pro | This study | To clone gfpmut3 into pUC18R6KT-mini-Tn7T-Km | ATAGGAATTCCTTCTCGAGCGACTGCACGGTGC |
p408/pGFPTn7R | This study | To clone gfpmut3 into pUC18R6KT-mini-Tn7T-Km | CAGAGCGCTTTTGAAGCTAATTCGATC |
p544 | [51] | To mutate hmsT | ACGTGGTACAACATGCTGACGGTTG |
p545 | [51] | To mutate hmsT | GAAGCAGCTCCAGCCTACACCATAATATCGTGCTGTCAGTAGACTAATAAATC |
p546 | [51] | To mutate hmsT | GGTCGACGGATCCCCGGAATGATTAACTCACTGAACATACGGACGCTCTATG |
p547 | [51] | To mutate hmsT | CTTCTCATCATCATCTGATGCGGCC |
p548 | [51] | To mutate hmsD | GCCAGTAATATCAATGCAGATATTCTGCG |
p549 | [51] | To mutate hmsD | GAAGCAGCTCCAGCCTACACCATAGATTGGTTTTTTATCGTGTTCATCGT |
p550 | [51] | To mutate hmsD | GGTCGACGGATCCCCGGAATGTGACATGCAGAAAATGCAAAACAAGCGTT |
p551 | [51] | To mutate hmsD | CGTTAGGACGCGGTGATAATAATGGCG |
p556 | This study | To verify hmsD mutation | AGCGCAGAATTCGTACCTAACGCCGATTCAACC |
p577 | This study | To verify hmsD mutation | ATCCACTTCATCCTGGCAGC |
p578 | This study | To verify hmsT mutation | CGCAAGACTCTCGCTTCTTTG |
p579 | This study | To verify hmsT mutation | TTCCACGTTTGTAGCAAACCC |