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Table 2 List of oligonucleotides used

From: Biovar-related differences apparent in the flea foregut colonization phenotype of distinct Yersinia pestis strains do not impact transmission efficiency

Oligonucleotide

Reference

Purpose

Sequence

p130

[35]

Amplify kanamycin cassette and check insertion

GTGTAGGCTGGAGCTGCTTC

p131

[35]

Amplify kanamycin cassette and check insertion

ATTCCGGGGATCCGTCGACC

p407/pGPTn7pro

This study

To clone gfpmut3 into pUC18R6KT-mini-Tn7T-Km

ATAGGAATTCCTTCTCGAGCGACTGCACGGTGC

p408/pGFPTn7R

This study

To clone gfpmut3 into pUC18R6KT-mini-Tn7T-Km

CAGAGCGCTTTTGAAGCTAATTCGATC

p544

[51]

To mutate hmsT

ACGTGGTACAACATGCTGACGGTTG

p545

[51]

To mutate hmsT

GAAGCAGCTCCAGCCTACACCATAATATCGTGCTGTCAGTAGACTAATAAATC

p546

[51]

To mutate hmsT

GGTCGACGGATCCCCGGAATGATTAACTCACTGAACATACGGACGCTCTATG

p547

[51]

To mutate hmsT

CTTCTCATCATCATCTGATGCGGCC

p548

[51]

To mutate hmsD

GCCAGTAATATCAATGCAGATATTCTGCG

p549

[51]

To mutate hmsD

GAAGCAGCTCCAGCCTACACCATAGATTGGTTTTTTATCGTGTTCATCGT

p550

[51]

To mutate hmsD

GGTCGACGGATCCCCGGAATGTGACATGCAGAAAATGCAAAACAAGCGTT

p551

[51]

To mutate hmsD

CGTTAGGACGCGGTGATAATAATGGCG

p556

This study

To verify hmsD mutation

AGCGCAGAATTCGTACCTAACGCCGATTCAACC

p577

This study

To verify hmsD mutation

ATCCACTTCATCCTGGCAGC

p578

This study

To verify hmsT mutation

CGCAAGACTCTCGCTTCTTTG

p579

This study

To verify hmsT mutation

TTCCACGTTTGTAGCAAACCC