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Fig. 1 | Parasites & Vectors

Fig. 1

From: Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis

Fig. 1

Preparation of transgenic Eimeria tenella Wis parasites expressing EmIMP1. a Simplified representation of the plasmid used for E. tenella transfection coding for the EmIMP1 protein. Scissors represent the location of the XbaI restriction site used for transgene insertion. F and R represent the primers used to confirm transgene transcription by reverse transcription (RT)-PCR. b Detection of EmIMP1-mCherry transcripts in cDNA isolated from stable transgenic populations by RT-PCR. A single band of ~ 0.9 kb was obtained from E. tenella populations expressing EmIMP1 (Et[EmIMP1]), but not from the wild-type vector (EtW). The construct used for parasite transfection was included as a positive control. A non-template control (NTC) was also included. c Detection of EmIMP1-mCherry expression by confocal microscopy. The mCitrine was expressed as a cytosolic protein and used to select transgenic parasites by flow cytometry, whereas the EmIMP1-mCherry fusion protein was secreted into the sporocyst cavity and anchored onto the sporozoite surface [17]. Scale-bars: 10 µm

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