Skip to main content

Table 1 Experimental design for vaccine trial

From: Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis

Group

Abbreviation

Vaccine

Immunisation protocol

Challenge (E. maxima W)

n PRd (cages)

n LSe (pens)

n BWGf (pens)

Day 2

Day 8

Day 14

Day 29

Culled at day 35

Culled at day 35

Culled at day 41

1

H2O-H2O

H2O

H2O

H2O

H2O

H2O

7

11

H2O

6

2

H2O-Emax

H2O (no protection)

H2O

H2O

H2O

10,000

7

11

300

6

3

Emax-Emax

E. maxima W (‘full’ protection)

100

500

3000

10,000

7

11

300

6

4

Et[GPI]-Emax

Et[GPI]a,b (empty vector)

100

500

3000

10,000

7

11

300

6

5

Et[A]-Emax

Et[EmAMA1]a

100

500

3000

10,000

7

11

300

6

6

Et[A + I]-Emax

Et[EmAMA1] + Et[EmIMP1]a,c

100

500

3000

10,000

7

11

300

6

  1. aFACS enriched transgenic E. tenella parasites
  2. bEimeria tenella Wis parasites expressing the signal peptide of the EtMIC2 protein and the glycosylphosphatidylinositol (GPI) anchor of the EtSAG1 protein
  3. cEqual proportions of EmAMA1 and EmIMP1-expressing parasites were used for vaccination
  4. dNumber of birds used to quantify parasite replication
  5. eNumber of birds used to assess lesion scores
  6. fNumber of birds used to quantify body weight gains