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Table 1 Experimental design for vaccine trial

From: Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis

Group Abbreviation Vaccine Immunisation protocol Challenge (E. maxima W) n PRd (cages) n LSe (pens) n BWGf (pens)
Day 2 Day 8 Day 14 Day 29 Culled at day 35 Culled at day 35 Culled at day 41
1 H2O-H2O H2O H2O H2O H2O H2O 7 11
H2O 6
2 H2O-Emax H2O (no protection) H2O H2O H2O 10,000 7 11
300 6
3 Emax-Emax E. maxima W (‘full’ protection) 100 500 3000 10,000 7 11
300 6
4 Et[GPI]-Emax Et[GPI]a,b (empty vector) 100 500 3000 10,000 7 11
300 6
5 Et[A]-Emax Et[EmAMA1]a 100 500 3000 10,000 7 11
300 6
6 Et[A + I]-Emax Et[EmAMA1] + Et[EmIMP1]a,c 100 500 3000 10,000 7 11
300 6
  1. aFACS enriched transgenic E. tenella parasites
  2. bEimeria tenella Wis parasites expressing the signal peptide of the EtMIC2 protein and the glycosylphosphatidylinositol (GPI) anchor of the EtSAG1 protein
  3. cEqual proportions of EmAMA1 and EmIMP1-expressing parasites were used for vaccination
  4. dNumber of birds used to quantify parasite replication
  5. eNumber of birds used to assess lesion scores
  6. fNumber of birds used to quantify body weight gains