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Table 6 H2O2 selection experiment of the H2O2-resistant strain Ls V: design and results (% affected lice)

From: Candidate genes for monitoring hydrogen peroxide resistance in the salmon louse, Lepeophtheirus salmonis

F H2O2 exposure data Louse instar (n) % affected lice
F1 FBT (4): 1500 ppm, 15–20 min, 11 °C Pre-adult I-II (250) 8
BIO: 2000 ppm, 30 min, 11 °C Pre-adult II – adult males (180) 33
F2 FBT (4): 1500 ppm, 15–20 min, 8.5 °C Pre-adult I-II (150) 7
BIO: 2500 ppm, 30 min, 8.5 °C Pre-adult II – adult males (110) 50
F3 Not selected with H2O2
F4 FBT (16): 1500 ppm, 15–20 min, 8.5 °C Pre-adult I-II (360) 8.3
BIO: 2500 ppm, 30 min, 11 °C Pre-adult II – adult males (312) 63
F5 Six-dose bioassay Pre-adult II – adult males
  1. Notes: F: lice generation (this F2 generation is not the same as the F2 generation from the crossing and RNAseq experiments). H2O2 exposure type and data: FBT (fish bath treatment), lice treated on-fish using a bath treatment methodology (number of fish used in parentheses); BIO (bioassay selection), lice treated off-fish using a bioassay methodology; H2O2 concentration, exposure time, water temperature. Instar: louse developmental stage; n: number (approximately) of lice used in each selection event (males and females together) in parentheses. Selection on generation 3 (F3) could not be performed due to low lice numbers. F5 was not selected with H2O2; this generation was used to test the H2O2 sensitivity after selection using a six-dose bioassay (see bioassay details in Table 5)