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Table 6 H2O2 selection experiment of the H2O2-resistant strain Ls V: design and results (% affected lice)

From: Candidate genes for monitoring hydrogen peroxide resistance in the salmon louse, Lepeophtheirus salmonis

F

H2O2 exposure data

Louse instar (n)

% affected lice

F1

FBT (4): 1500 ppm, 15–20 min, 11 °C

Pre-adult I-II (250)

8

BIO: 2000 ppm, 30 min, 11 °C

Pre-adult II – adult males (180)

33

F2

FBT (4): 1500 ppm, 15–20 min, 8.5 °C

Pre-adult I-II (150)

7

BIO: 2500 ppm, 30 min, 8.5 °C

Pre-adult II – adult males (110)

50

F3

Not selected with H2O2

–

–

F4

FBT (16): 1500 ppm, 15–20 min, 8.5 °C

Pre-adult I-II (360)

8.3

BIO: 2500 ppm, 30 min, 11 °C

Pre-adult II – adult males (312)

63

F5

Six-dose bioassay

Pre-adult II – adult males

–

  1. Notes: F: lice generation (this F2 generation is not the same as the F2 generation from the crossing and RNAseq experiments). H2O2 exposure type and data: FBT (fish bath treatment), lice treated on-fish using a bath treatment methodology (number of fish used in parentheses); BIO (bioassay selection), lice treated off-fish using a bioassay methodology; H2O2 concentration, exposure time, water temperature. Instar: louse developmental stage; n: number (approximately) of lice used in each selection event (males and females together) in parentheses. Selection on generation 3 (F3) could not be performed due to low lice numbers. F5 was not selected with H2O2; this generation was used to test the H2O2 sensitivity after selection using a six-dose bioassay (see bioassay details in Table 5)
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Parasites & Vectors

ISSN: 1756-3305

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