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Fig. 2 | Parasites & Vectors

Fig. 2

From: Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti

Fig. 2

Schematic representation of the transformation constructs. All constructs consisted of the mariner MosI right arm (Mos R. A.) and left arm (Mos L. A.) and carried the marker gene cassette PolyUbDsRed. The polyubiquitin promoter is expected to express DsRED in all stages of mosquito development and most tissues. The dotted lines represent sequence from the plasmid backbone. The bZip-mNG construct encodes a gene cassette composed of the bZip1 promoter driving the expression of the mNeon Green fluorescent protein (mNG). bZip-tTA and bZip-tTA-mNG constructs also carry a gene composed of the bZip1 promoter region, but driving the expression of the transactivator (tTA) from the Tet-off system; construct bZip-tTA-mNG expresses along with the tTA, the self-cleaving peptide T2A coupled with the mNG fluorescent protein

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