Fig. 4

Expression profile of mNG in ovaries and embryos of bZip-mNG transgenic mosquitoes. a Ovaries from bZip-mNG P3 transgenic line (T) and wild type control (LVP) of NBF and 24, 48 and 72 h PBM females. b Accumulation of mNG in embryos of bZip-mNG P3, F1 and F2 transgenic lines at 0 h after they were laid. c Quantification of accumulated mNG and endogenous bZip1 transcripts by RT-qPCR in ovaries dissected from transgenic bZip-mNG P3, F1 and F2 and LVP females NBF, 24, 48 and 72 h post-blood-meal and embryos 0, 2, 4 and 6 h after being laid