Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti

Table 1 Microinjection results of bZip-mNG, bZip-tTA and bZip-tTA-mNG constructs

Construct	No. of embryos injected	Larvae hatching (%)^a	Survival (%)	No. of screened larvae^b	No. of DsRed positive larvae	No. of transgenic lines	Transformation rate (%)^c
bZip-mNG	2126	424 (20)	415 (19)	20,484	104	3	1.4
bZip-tTA	1825	634 (35)	634 (35)	30,365	14	3	0.9
bZip-tTA-mNG	2065	399 (19)	370 (18)	50,518	50	1	0.5

^aPercentage of injected embryos that hatched into L3-L4 larvae
^bPercentage of injected embryos that hatched into adults
^cPercentage of independent transformed lines generated per fertile adult (assumes ~ 50% fertility)

ISSN: 1756-3305