Fig. 5

Effect of FgESPs on development and immune functions of buffalo DCs after DNMT1- or TET1-knockdown. a Transcriptional profile of DC development and immune function associated markers was determined by qRT-PCR. The data is represented as the relative mRNA expression level, calculated by 2^−ΔΔCq method. b Production of typical cytokines involved in buffalo DCs development and immune functions was measured by commercial ELISA kits. Columns in red and blue represent the normal DCs treated with PBS control (red) and FgESP (blue) alone, respectively, while the yellow and green columns represent the DNMT1 siRNA or TET1 siRNA-transfected DCs (with RNAi on corresponding targets) that were treated with the PBS control (yellow) and FgESP (green) alone, respectively. Representative histograms from three independent experiments are shown. All data are presented as the mean ± standard error (SE) of triplicate measurements. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001