Fig. 3

EtEsp expression at different developmental stages of E. tenella. a qPCR of EtEsp at different developmental stages of E. tenella. Bars with different letters indicate significant differences (P < 0.05) and the error bars indicate standard deviations. b Western blot showing EtEsp at different developmental stages, probed with rabbit anti-rEtEsp serum or mouse monoclonal anti-α-tubulin antibody. c The densitometric intensity of western blot images was analyzed using ImageJ software. Bars with different letters indicate significant differences (P < 0.05) and the error bars indicate standard deviations. Abbreviations: UO, unsporulated oocysts; SO, sporulated oocysts; Spz, sporozoites; sMrz, second-generation merozoites