Fig. 7

Immunofluorescent localization of EtEsp at different developmental stages of E. tenella. Parasites were immuno-stained with anti-rEtEsp antibody. a Sporozoites (Spz) were incubated in PBS (a1 permeabilized sporozoites, a2 non-permeabilized sporozoites). b Sporocysts (Sporo) were incubated in PBS (b1 permeabilized sporocysts, b2 non-permeabilized sporocysts). c Sporozoites (Spz) were incubated in complete medium (CM) for 2 h at 41 °C. d, e, intracellular sporozoites (iSpz) at 2 h and 12 h post-infection, respectively. f Trophozoites (iTropho) at 24 h post-infection. g, h Immature schizonts (iSc) at 48 and 60 h post-infection, respectively. i Mature schizonts (mSc) at 68 h post-infection. j First-generation merozoites (fMrz) at 72 h post-infection. k Second-generation merozoites (sMrz) in PBS