Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China

Parasites & Vectors

Table 4 Tandem repeat analysis of TRAP gene fragments

Haplotype	No. of positive blood samples	No. of repeats	Position	Consensus pattern	Consensus size (bp)	No. of copy	Percent match
1	28 (N_Xi’an = 17, N_Hanzhong = 11)	–	–	–	–	–	–
2	3 (N_Xi’an = 1, N_Hanzhong = 2)	3	795–845	GGAGGA	6	8.5	93
			796–845	GAGGAAGAGGAA	12	3.9	82
			795–845	GGAGGAGGAGGAAGAGGAAGA	21	2.4	96
3	42 (N_Xi’an = 19, N_Hanzhong = 23)	5	796–857	GAGGAAGAGGAA	12	4.9	77
			796–857	GAGGAGGAGGAAGAGGAA	18	3.3	84
			795–857	GGAGGAGGAGGAAGAGGAAGA	21	3.1	84
			793–845	GCGGAGGAAGAGGAAGAGGAAGAGGAG	27	2.0	92
			823–857	GAGGAA	6	5.8	96
4	6 (N_Xi’an = 2, N_Hanzhong = 4	3	795–845	GGAGGAGGAAGAAGAGGAAGA	21	2.6	81
			793–845	GCGGAGGAAGAAGAAGAGGAAGAGGAG	27	2.0	88
			823–866	GAGGAA	6	5.8	96
5	59 (N_Xi’an = 36, N_Hanzhong = 23)	2	795–845	GGAGGAGGAGGAAGAGGAAGA	21	2.4	86
5	59 (N_Xi’an = 36, N_Hanzhong = 23)	2	793–845	GCGGAGGAAGAGGAAGAGGAAGAGGAG	27	2.0	92
6	6 (N_Xi’an = 5, N_Hanzhong = 1)	4	796–851	GAGGAAGAGGAA	12	4.4	76
			796–851	GAGGAGGAGGAAGAGGAA	18	2.9	84
			795–851	GGAGGAGGAGGAAGAGGAAGA	21	2.9	84
			793–845	GCGGAGGAAGAGGAAGAGGAAGAGGAG	27	2.0	92
7	22 (N_Xi’an = 17, N_Hanzhong = 5)	1	795–845	GGAGGAGGAGGAAGAGGAAGA	21	2.4	86
8	6 (N_Xi’an = 3, N_Hanzhong = 3)	4	795–845	GGAGGAGGAGGAAGAGGAAGA	21	2.4	86
			793–845	GCGGAGGAAGAGGAAGAGGAAGAGGAG	27	2.0	92
			823–866	GAGGAAGAGGAT	12	3.7	87
			823–857	GAGGAA	6	5.8	96
9	3 (N_Xi’an = 2, N_Hanzhong = 1)	3	795–845	GGAGGAGGAGGAAGAGGAAGA	21	2.4	86
			793–845	GCGGAGGAAGAGGAAGAGGAAGAGGAG	27	2.0	92
			823–866	GAGGAA	6	7.3	89

ISSN: 1756-3305