Fig. 7

Babesia microti-specific PCR performed with DNAs from blood samples (10 μl blood diluted to a final volume of 100 μl in PBS, final dilution 1:10) of B. microti-infected mice treated with tafenoquine (samples 1–5) or artesunate + tafenoquine (samples 6–10) at days 15 (a), 20 (b) and 27 (c) post-infection. The last dose of tafenoquine was given on day 8 post-infection, and parasitemia was undetectable by microscopy from day 9 onwards. Positive control (C+) was DNA from blood of a B. microti-infected mouse with patent parasitemia by microscopy, and negative control (C-) was DNA from blood of a non-infected mouse. In the PCR on day 20 (b), the positive control sample (4% microscopy parasitemia) was run at four different dilutions: 1:10 (PCR+), 1:100 (PCR+), 1:1000 (PCR+) and 1;10,000 (PCR-) in PBS. Abbreviation: M, molecular weight marker