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Fig. 2 | Parasites & Vectors

Fig. 2

From: The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba

Fig. 2

Cellular localization and SIRT deacetylase activity of AcSir2. a Intracellular localization of AcSir2 trophozoites (upper panel) transfected with pGAPDH-EGFP and pGAPDH-AcSir2-EGFP (green). Trophozoites were transferred into medium to induce encystation, incubated for 24 h (lower panel), and examined under a fluorescence microscope. Nuclei and mitochondria were visualized using DAPI staining (blue), and resulting merged images are presented. b SIRT deacetylase activity of AcSir2 in the nuclear extracts of the vector control (EGFP) and AcSir2-overexpressing trophozoites. Colorimetric assays were conducted with nuclear extracts following treatment with nicotinamide (500 μM) or salermide (500 μM). The data represent the mean ± standard deviation (SD). Significant group differences were evaluated with Tukey’s post-hoc multiple comparison tests. **P < 0.01 ***P < 0.001 and ****P < 0.0001. Scale-bars: 10 µm

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