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Fig. 3 | Parasites & Vectors

Fig. 3

From: The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba

Fig. 3

Effects of AcSir2 overexpression on the growth of A. castellanii trophozoites. a The total cell number of wild-type A. castellanii and cells transfected with pGAPDH-EGFP only and pGAPDH-AcSir2-EGFP plasmids 24, 48 and 72 h after incubation in PYG culture medium. The data represent the mean cell number ± standard deviation (SD) (**P < 0.001). b Effects of salermide on the proliferation of A. castellanii trophozoites. Trophozoites were incubated with various concentrations of salermide (a sirtuin inhibitor) or DMSO (a solvent control). The data represent the mean cell number 0, 24 and 48 h after incubation for each salermide or DMSO concentration. Significant group differences were evaluated with Tukey’s post-hoc multiple comparison tests. (**P < 0.01, ***P < 0.001 and ****P < 0.0001

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