Skip to main content
Fig. 6 | Parasites & Vectors

Fig. 6

From: The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba

Fig. 6

Inhibition of the encystation of A. castellanii with salermide treatment. a Ultrastructural changes following treatment with salermide in the encysting of A. castellanii. Encystation was induced by transferring cells into encystation medium containing DMSO (left) or 100 μM salermide for 24 h (right). The same amount of DMSO (as a solvent control) was also added to the encystation medium. The black arrowhead, empty arrowhead and black double-headed arrows indicate the exocyst wall, endocyst wall and intercyst space, respectively. b Transcriptional changes in cellulose synthase in encysting AcSir2-overexpressing cells. AcSir2-overexpressing trophozoites (0 h) and encysting cells at 24, 48 and 72 h after the induction of encystation were examined for transcriptional changes in cellulose synthase using qRT-PCR. The transcriptional levels of cellulose synthase were normalized to that of Acanthamoeba actin. c Effects of salermide on transcription levels in cellulose synthase during the encystation process. Trophozoites were transferred into encystation medium containing 100 μM of salermide, incubated for 24 h, and examined for transcriptional changes in cellulose synthase using qRT-PCR. The transcriptional levels of cellulose synthase in the salermide-treated cells were compared to those of DMSO-treated cells. **P < 0.01, ***P < 0.001 and ****P < 0.0001. Scale-bar: a, 1 μm

Back to article page