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Table 1 Designed primer sequences with the respective annealing temperature and amplicon size for gene amplification

From: Evaluation and utility of mitochondrial ribosomal genes for molecular systematics of parasitic nematodes

Target gene Primer name Sequence (5ʹ-3ʹ) Annealing temperature (°C) Amplicon size (bp)
12S rRNA 12S.C1.F GTGCCAGCTAYCGCGGTTA 55 460
12S.C1.R GRTGACGGGCRATATGTG
12S.C345.F GTWCCAGAATAATCGGMTA 46
12S.C345.R ATTGAYGGATGRTTTGTRC
16S rRNA 16S.C1.F ACGAGAAGACCCTRGRAAYT 50 240
16S.C1.R GRTYTAAACTCAAATCACG
16S.C345.F AAGATAAGTCTTYGGAARYT 45
16S.C345.R GAAYTAAACTAATATCAM G