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Fig. 1 | Parasites & Vectors

Fig. 1

From: Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens

Fig. 1

Performance of the TgSAG1bio bead-based multiplex assay in experimentally Toxoplasma gondii-infected chickens. Median fluorescence intensities (MFI) in sera collected from non-inoculated chickens (green, n = 23) and inoculated chickens (n = 66). Results of inoculated chickens are split into inoculated and negative in direct detection by magnetic capture real time PCR (InocNeg, orange, n = 21) or inoculated and positive in direct detection (InocPos, red, n = 45). Data are displayed as box plots of median (line), 25th and 75th percentile (box), the 1.5-fold interquartile ranges (whiskers) and outliers (dots). The cut-off to separate reaction of inoculated from non-inoculated chickens is indicated by a red line (MFI = 322.5). The cut-off used within the group of inoculated chickens to separate the reactions of birds that tested positive by direct detection is indicated by a black line (MFI = 3092). In addition to the specific reactions (TgSAG1bio), results for internal controls, i.e. negative (chicken albumin; background) and positive controls (chicken IgY), are shown

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