Fig. 2
From: Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens
Performance of the TgSAG1bio-BBMA in chickens naturally exposed to Toxoplasma gondii. Median fluorescence intensity (MFI) in sera collected from reference-negative chickens (green, n = 378), reference-positive chickens (red, n = 50) and chickens that could not be assorted to the reference population (NA, n = 18) because of diverging results in serological reference assays (ELISA, IFAT and MAT). Data are displayed as box plots showing median (line), 25th and 75th percentile (box), the 1.5-fold interquartile ranges (whiskers) and outliers (dots). The cut-off applied is indicated by a red line (MFI = 322.5). In addition to the specific reactions (TgSAG1bio), also internal control reactions, i.e. negative control (chicken albumin) and a positive control reaction (chicken IgY) are shown. Notes: All negative reference chickens with background reactions MFI > 322.5 tested negative in the TgSAG1bio-BBMA. Two positive reference chickens with background reactions MFI > 322.5 were regarded as negligible, because their TgSAG1bio-BBMA reactions were at least 7-times higher and exceeded MFI 9000