Fig. 2

Amino acid sequence alignment of the conserved PEWY region of cytochrome b of six ovine Babesia isolates, Theileria parva, Toxoplasma gondii, B. microti, Plasmodium falciparum, Saccharomyces cerevisiae, Bos taurus and B. duncani. The alignment was constructed using MegAlign. The number 275 indicates to the residues at position Leu²⁷⁵ of S. cerevisiae. The residue at position 275 is a key determinant of the efficacy of ligand (atovaquone) binding to the bc1 complex. Arrows indicate amino acid positions altered in atovaquone-resistant parasites. Red horizontal lines indicate positions that are completely conserved in all species