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Table 4 Main resistance-related mutations and drug binding residues of cytochrome b in Saccharomyces cerevisiae, Plasmodium falciparum, B. microti, B. duncani and six ovine Babesia isolates

From: Insights into the phylogenetic relationships and drug targets of Babesia isolates infective to small ruminants from the mitochondrial genomes

Target protein Drug Amino acid changes conferring drug resistance in the yeast numbering system S. cerevisiae residues P. falciparum residues B. microti residues B. duncani residues BspXJ/DH residues BmLT/TZ residues BmNX/HB residues
Qo site of the bc1 complex Atovaquone Ile269→Met, Phe278→Ile, Phe278→Ala, Tyr279→Cys, Tyr279→Ser, Leu282→Val, Leu275→Phe Glu272, Leu275, Phe278, Tyr279 Glu261, Phe267, Tyr268 Glu265, Leu268, Phe271, Tyr272 Glu257, Leu260, Phe263, Tyr264 Glu259, Leu262, Phe265, Tyr266 Glu259, Leu262, Phe265, Tyr266 Glu259, Leu262, Phe265, Tyr266
Stigmatellin Leu275→Phe Glu272 Glu261 Glu265 Glu257 Glu259 Glu259 Glu259
Myxothiazol Leu275→Phe Leu275 Leu268 Leu260 Leu262 Leu262 Leu262
ELQ-110 Met139, Gly143, Glu272 Met139, Gly143, Glu272 Met133, Gly137, Glu261 Met134, Gly138, Glu265 Met126, Gly130, Glu257 Met128, Gly132, Glu259 Met128, Gly132, Glu259 Met128, Gly132, Glu259
Qi site of the bc1 complex Antimycin A Asn31, Ser34, Gly37, Met221, Phe225, Lys228, Asp229 Ser34, His202, Lys228, Asp229 His192, Asp218 His193,
Asp220
His185, Asp212 His187, Asp214 His187, Asp214 His187, Asp214
ELQ-300 Ile26→Leu, Asp229 Ile26, Asp229 Ile22, Asp218 Asp220 Ile15, Asp212 Asp214 Asp214 Asp214
Qi and Qo sites of the bc1 complex NQNO Trp30, Asn31, Gly33, Gly37, His204, Ser206, Met221, Phe225 His202, Ser206, Asp229 His192, Ser196, Asp218 His193,
Ser197, Asp220
His185, Ser189, Asp212 His187,
Ser191, Asp214
His187, Ser191, Asp214 His187, Ser191, Asp214