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Fig. 2 | Parasites & Vectors

Fig. 2

From: TgROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection

Fig. 2

Extracellular TgROP18 resulted in STAT3-Y705 phosphorylation in HaCaT cells in a dose-dependent manner without affecting the level of total STAT3. a HaCaT cells were infected with indicated parasites, the control group was treated with the IL-20 vehicle solution (BSA-PBS), the positive control was stimulated with IL-20. a, c, e The total proteins of HaCaT cells were extracted and subjected to western blot (WB). b, d, f Densitometric quantitation of each band in a, c and e was applied using Image J. c HaCaT cells were treated with indicated amount of recombinant GST and GST-ROP18, and elution buffer was used as control. d HaCaT cells were infected with gradient MOI of indicated parasite, or uninfected as control. Statistical analysis was conducted by Kruskal-Wallis H-test with Bonferroni correction (*P < 0.05, *P < 0.01 and ***P < 0.001). Error bars show the standard errors (SD)

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