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Fig. 5 | Parasites & Vectors

Fig. 5

From: TgROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection

Fig. 5

Detection of the activation of IL20R-JAK/STAT3 pathway in HaCaT cells treated with recombinant TgROP18 protein. a Transcription and expression of IL20RB in HaCaT relative to HFF was detected by qRT-PCR and Western blot. b COS-7, HaCaT, and HFF cells were treated with IL-20, recombinant GST, or recombinant GST-ROP18 for 30 min, and elution buffer treated cells were used as negative control. STAT3-pY705 level was determined by WB. c, f Densitometric quantitation of each band in B or E was applied using Image J. d, e HaCaT cells were infected with indicated parasites for the indicated times. TNF-α transcription and translation was detected by qRT-PCR and WB. Statistical analysis was performed with the Kruskal-Wallis H-test with Bonferroni correction (**P < 0.01)

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