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Fig. 3 | Parasites & Vectors

Fig. 3

From: Identification and validation of a commercial cryopreservation medium for the practical preservation of Dirofilaria immitis microfilaria

Fig. 3

Analysis of in vivo infectivity of A. aegypti mosquitoes with cryopreserved D. immitis microfilariae (mf). a Survival of mosquitoes infected with mf cryopreserved in PVP-DMSO, CultureSure and LaboBanker and non-cryopreserved control (Fresh) over 13 d post-infection (dpi). The survival rate on 13 dpi was statistically compared using a Chi-square test. Different letters (a, b) indicate significant differences (P < 0.05). b The number of third-stage larvae (L3) in the mosquitoes on 13 dpi. Each plot shows the L3 of individual mosquitoes. Error bars = mean ± SD. The L3 were statistically compared using Welch’s t-test. Significant differences (P < 0.05) were observed between different letters (a, b); † indicate marginal differences

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