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Fig. 3 | Parasites & Vectors

Fig. 3

From: Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis

Fig. 3

Identification of Leishmania species from scraping/exudate clinical samples. PCR amplification of Leishmania genomic DNA from lesion scraping/exudate using the newly developed universal primers UNIL-IR-P and UNIL-IR-M subjected to electrophoresis on a 1.5% agarose gel. Lane 1: amplicon of L. major MRHO/IR/75/ER reference strain; Lane 2: amplicon of L. major culture+/smear- samples; Lane 3: amplicon of L. major culture-/smear+ samples. Lane 4: amplicon of L. tropica MHOM/SU/74/K27 reference strain; Lane 5: amplicon of L. tropica culture+/smear+ samples; Lane 6: amplicon of culture-/smear-samples; Lane 7: amplicon of L. infantum MCAN/IR/96/LON49 reference strain; Lane 8: non-template negative control. Lane L: 100 bp DNA ladder

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Parasites & Vectors

ISSN: 1756-3305

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